Journal: International Journal of Molecular Sciences

Article Title: High-Salt Diet Accelerates Neuron Loss and Anxiety in APP/PS1 Mice Through Serpina3n

doi: 10.3390/ijms252111731

Figure Lengend Snippet: HS diet induces neuron reduction in AD mice. ( A ) Thioflavin T staining was used to detect S-plaque in mice brains. No S-plaque was observed in the HS/ND-treated wt mice; n = 5 in each group. Scale bar = 50 μm. ( B , C ) Nissl staining was performed to detect the neuron number of HS/ND-treated wt mice. Neuron numbers were counted in the CA1 region of the hippocampus in each group. Scale bar = 50 μm. ( D , E ) Thioflavin T staining was used to detect S-plaque in the brains of HS/ND-treated AD mice. The number of S-plaques in parietal cortex, hippocampus, and prefrontal cortex was counted; n = 5 in each group. Scale bar = 50 μm. ( F ) Representative Nissl staining of hippocampus in each group. CA1 regions are magnified on the right. The immunohistochemistry for NeuN in the hippocampus of each group is also shown. Between the two green dotted lines is the neurons in the CA1 region. Scale bar = 50 μm. ( G ) The Nissl body-positive neuron numbers were counted from the CA1 region of brain slides from five mice in each group; n = 5 in each group. The scatter bar chart shows the integrated density of NeuN-positive particles in each brain slide from five mice, which was measured using Image J software; n = 5 in each group. ( H ) Immunohistochemistry for syn1 staining in the hippocampus of each group. Between the two green dotted lines is the neurons in the Or, Rad, and Mol regions; scale bar = 20 μm. ( I ) The scatter bar chart shows the integrated density of Syn1-positive particles from Or, Rad, and Mol, respectively. Each brain slide from five mice was measured using Image J software; n = 5 in each group. ( J , K ) Western blot analysis for caspase 3, Bax, Syn1, and β-tubulin 3; β-actin was used as an internal control. The data are the mean ± SE; n = 4 biologically independent animals. Blue represent the ND treated mice, red represent the HS treated mice.

Article Snippet: The sections were incubated with rabbit anti-NeuN (1:500, 14H6L24, Invitrogen, CA, USA) or mouse anti-syn1 (1:200, 7H10G6, Novus, CA, USA) overnight at 4 °C.

Techniques: Staining, Immunohistochemistry, Software, Western Blot, Control